FAO FORESTRY PAPER 118
Biotechnology in forest tree improvement with special reference to developing countries |
by
Russell Haines
FAO André Mayer Fellow
The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.
M-31
ISBN 92-5-103490-7
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Food and Agriculture Organization of the United Nations
Rome © FAO 1994
This review, prepared under an André Mayer Research Fellowship awarded by FAO in 1991, reports on the present state and future potential of biotechnologies in forest tree breeding, with special reference to their application in developing countries.
It is possible to increase greatly the environmental adaptation and productivity of forest plantations and of trees planted outside the forest in direct support to agriculture or for protection purposes, through selection and genetic improvement programmes. Much hope has been pinned on rapid improvement in adaptation and yield by the use of traditional, and new and advanced, technologies in genetics and forest tree breeding based on some documented success stories and optimistic projections.
The document confirms that new technologies offer possibilities which will greatly facilitate the work of the tree breeder in the future. It stresses, however, that the potential of the powerful new tools which biotechnology offers can only be realized if applied within existing, sound, tree improvement programmes and if based on basic, biological knowledge on the species concerned.
The document is aimed at tree breeders and professional foresters with some prior knowledge of the subject, although a brief Glossary of scientific terms used, is given at the end of the document. It is hoped that the information provided will help colleagues in FAO Member Nations to assess the actual and potential uses and limitations of biotechnologies in forest tree breeding, and that it will promote the sound application of the new and exciting tools that such technologies offer.
J.P. Lanly
Director
Forest Resources Division
Forestry Department
The present document is the result of work carried out by FAO André Mayer Fellow, Russell Haines of the Queensland Forest Service (Australia).
The Association Forêts-Cellulose (AFOCEL), Nangis, France, acted as Host Institute for the 14 month study, and provided facilities as well as administrative, technical and scientific support to the Fellow throughout the study. The assistance of M. M. Armitano-Grivel, Mme E. Brouzheng Mme A. Lecointe, Mme C. Hubert and Dr J.-N. Ruaud, is especially acknowledged.
Thanks also go to the Queensland Forest Service (QFS) for releasing the Fellow for the time of the study, and for financial and technical support in its course. In particular, thanks are due to Dr. D.G. Nikles and Steven Walker of the QFS.
The Fellowship included wide-ranging travel in Europe and in North and South America. Institutes in Asia and Australia provided inputs during travel carried out by the Fellow prior to, and immediately following, the FAO Fellowship. Numerous institutes and individuals in all regions of the world provided ideas and information, and assisted in the development of the study through discussions and written inputs. Our thanks go to all of these friends and colleagues who are too numerous to list, with special reference to J.Chaparro, G.Dale, R.Griffin, P.Kanowski, D.G. Nikles, C.Palmberg-Lerche, A.Simons, O. Souvannavong, S.Strauss and, last but not least, Jean and Sally Paquet.
The study was coordinated by Ms. C. Palmberg-Lerche, Chief Forest Resources Development Branch, Forest Resources Division of FAO's Forestry Department in Rome, with the assistance of Mr. O. Souvannavong and Ms. A. Thomsen of the same Division. Colleagues in FAO's Agriculture Department, and more specifically in its Plant Production and Protection Division and in its Research and Technology Development Division, also provided technical support to the study, under the over-all administrative guidance of the Fellowships Group of the Agricultural Operations Division.
Hyperlinks to non-FAO Internet sites do not imply any official endorsement of or responsibility for the opinions, ideas, data or products presented at these locations, or guarantee the validity of the information provided. The sole purpose of links to non-FAO sites is to indicate further information available on related topics.
This electronic document has been scanned using optical character recognition (OCR) software. FAO declines all responsibility for any discrepancies that may exist between the present document and its original printed version.
2. CURRENT STATUS OF TREE IMPROVEMENT
Elements of a Genetic Improvement Program
1. Tropical and sub-tropical eucalypts
7. Long rotation temperate conifers
Tree Improvement in Developed Countries
Tree Improvement in Developing Countries
3. CRYOPRESERVATION AND IN VITRO STORAGE
Principles and Experimental Achievements
Applications in Forest Tree Improvement
Restriction Fragment Length Polymorphisms (RFLPs)
Randomly Amplified Polymorphic DNAs (RAPDs)
Quantification of Genetic Diversity
Genotype Verification and Delineation
Taxonomic and Phylogenetic Studies
Applications in Forest Tree Improvement
Assessment of Genetic Diversity
Assembly of Breeding Populations
Genotype Verification and Delineation
Taxonomy and Phylogenetic Studies
Biological Studies (Pollen Contamination, Mating System etc)
Principles and Experimental Achievements
Selection for Disease Resistance
Selection for Herbicide Tolerance
Selection for Tolerance to Metals
Selection for Tolerance to High Temperature
Selection for Tolerance to Low Temperature
Selection for Tolerance to Water Stress
Applications in Forest Tree Improvement
Toxin Gene from Bacillus thuringiensis (B.t)
Bacterial and Fungal Resistance
Self-Incompatibility and Cross-Compatibility
Integration into Tree Breeding Programmes
Principles and Experimental Achievements
Tolerance to Salt and Other Stresses
Application to Forest Tree Improvement
Principles and Experimental Achievements
Application to Forest Tree Improvement
Principles and Experimental Achievements
Application to Forest Tree Improvement
Principles and Experimental Achievements
Application to Forest Tree Improvement
Principles and Experimental Achievements
Application to Forest Tree Improvement
Field Performance of Propagules
Applicability to Adult Material
Compatibility with Culture Storage
Proportion of genetypes Responding
12. IN VITRO CONTROL OF THE MATURATION STATE
Principles and Experimental Achievements
Application to Forest Tree Improvement
The Role of Juvenility in Clonal Forestry
The Effect of Generation Interval on Genetic Gain
The Status of Tree Improvement
Biotechnologies and their Applications to Tree Improvement
Cryopreservation and In Vitro Storage
In Vitro Control of the Maturation State
Biotechnology and Tree Improvement in Developing Countries
Cover photo:
Linkage map of the Pinus elliottii x P. caribaea F1 hybrid.
Photo: G. Dale