FAO FORESTRY PAPER     118

Cover
Biotechnology in forest tree improvement
with special reference to developing countries

TABLE OF CONTENTS


by
Russell Haines
FAO André Mayer Fellow


The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.


M-31
ISBN 92-5-103490-7


All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying or otherwise, without the prior permission of the copyright owner. Applications for such permission, with a statement of the purpose and extent of the reproduction, should be addressed to the Director, Publications Division, Food and Agriculture Organization of the United Nations, Viale delle Terme di Caracalla, 00100 Rome, Italy.

Food and Agriculture Organization of the United Nations
Rome © FAO 1994

FOREWORD

This review, prepared under an André Mayer Research Fellowship awarded by FAO in 1991, reports on the present state and future potential of biotechnologies in forest tree breeding, with special reference to their application in developing countries.

It is possible to increase greatly the environmental adaptation and productivity of forest plantations and of trees planted outside the forest in direct support to agriculture or for protection purposes, through selection and genetic improvement programmes. Much hope has been pinned on rapid improvement in adaptation and yield by the use of traditional, and new and advanced, technologies in genetics and forest tree breeding based on some documented success stories and optimistic projections.

The document confirms that new technologies offer possibilities which will greatly facilitate the work of the tree breeder in the future. It stresses, however, that the potential of the powerful new tools which biotechnology offers can only be realized if applied within existing, sound, tree improvement programmes and if based on basic, biological knowledge on the species concerned.

The document is aimed at tree breeders and professional foresters with some prior knowledge of the subject, although a brief Glossary of scientific terms used, is given at the end of the document. It is hoped that the information provided will help colleagues in FAO Member Nations to assess the actual and potential uses and limitations of biotechnologies in forest tree breeding, and that it will promote the sound application of the new and exciting tools that such technologies offer.

J.P. Lanly
Director
Forest Resources Division
Forestry Department

ACKNOWLEDGEMENTS

The present document is the result of work carried out by FAO André Mayer Fellow, Russell Haines of the Queensland Forest Service (Australia).

The Association Forêts-Cellulose (AFOCEL), Nangis, France, acted as Host Institute for the 14 month study, and provided facilities as well as administrative, technical and scientific support to the Fellow throughout the study. The assistance of M. M. Armitano-Grivel, Mme E. Brouzheng Mme A. Lecointe, Mme C. Hubert and Dr J.-N. Ruaud, is especially acknowledged.

Thanks also go to the Queensland Forest Service (QFS) for releasing the Fellow for the time of the study, and for financial and technical support in its course. In particular, thanks are due to Dr. D.G. Nikles and Steven Walker of the QFS.

The Fellowship included wide-ranging travel in Europe and in North and South America. Institutes in Asia and Australia provided inputs during travel carried out by the Fellow prior to, and immediately following, the FAO Fellowship. Numerous institutes and individuals in all regions of the world provided ideas and information, and assisted in the development of the study through discussions and written inputs. Our thanks go to all of these friends and colleagues who are too numerous to list, with special reference to J.Chaparro, G.Dale, R.Griffin, P.Kanowski, D.G. Nikles, C.Palmberg-Lerche, A.Simons, O. Souvannavong, S.Strauss and, last but not least, Jean and Sally Paquet.

The study was coordinated by Ms. C. Palmberg-Lerche, Chief Forest Resources Development Branch, Forest Resources Division of FAO's Forestry Department in Rome, with the assistance of Mr. O. Souvannavong and Ms. A. Thomsen of the same Division. Colleagues in FAO's Agriculture Department, and more specifically in its Plant Production and Protection Division and in its Research and Technology Development Division, also provided technical support to the study, under the over-all administrative guidance of the Fellowships Group of the Agricultural Operations Division.


Hyperlinks to non-FAO Internet sites do not imply any official endorsement of or responsibility for the opinions, ideas, data or products presented at these locations, or guarantee the validity of the information provided. The sole purpose of links to non-FAO sites is to indicate further information available on related topics.

This electronic document has been scanned using optical character recognition (OCR) software. FAO declines all responsibility for any discrepancies that may exist between the present document and its original printed version.


TABLE OF CONTENTS

FOREWORD

ACKNOWLEDGEMENTS

SUMMARY

1.    INTRODUCTION

2.    CURRENT STATUS OF TREE IMPROVEMENT

Elements of a Genetic Improvement Program

Status of Tree Improvement

Industrial species

1.   Tropical and sub-tropical eucalypts

2.   Cold-tolerant eucalypts

3.   Other tropical hardwoods

4.   Poplars and willows

5.   Other temperate hardwoods

6.   Medium rotation conifers

7.   Long rotation temperate conifers

Non-industrial species

Tree Improvement in Developed Countries

Tree Improvement in Developing Countries

3.    CRYOPRESERVATION AND IN VITRO STORAGE

Principles and Experimental Achievements

In Vitro Storage

Cryopreservation

Applications in Forest Tree Improvement

Germplasm Preservation

Maintenance of Juvenility

Transport of Germplasm

Conclusions

4.    MOLECULAR MARKERS

Methods

Isozymes

Restriction Fragment Length Polymorphisms (RFLPs)

Randomly Amplified Polymorphic DNAs (RAPDs)

Microsatellites

Principles and Achievements

Quantification of Genetic Diversity

Genotype Verification and Delineation

Taxonomic and Phylogenetic Studies

Biological Studies

Genetic Fingerprinting

Marker Assisted Selection

Applications in Forest Tree Improvement

Assessment of Genetic Diversity

Gene Conservation

Assembly of Breeding Populations

Genotype Verification and Delineation

Taxonomy and Phylogenetic Studies

Biological Studies (Pollen Contamination, Mating System etc)

Genetic Fingerprinting

Marker Assisted Selection

Conclusions

5.    IN VITRO SELECTION

Principles and Experimental Achievements

Selection for Disease Resistance

Selection for Herbicide Tolerance

Selection for Salt Tolerance

Selection for Tolerance to Metals

Selection for Tolerance to High Temperature

Selection for Tolerance to Low Temperature

Selection for Tolerance to Water Stress

Cross Tolerance to Stresses

Selection for Other Traits

Applications in Forest Tree Improvement

6.    GENETIC ENGINEERING

Strategies

General Techniques

Introduction of Genes

Promoters

Marker Genes

Targeted Traits

Insect Resistance

Toxin Gene from Bacillus thuringiensis (B.t)

Proteinase Inhibitors

Other Genes

Virus Resistance

Bacterial and Fungal Resistance

Nematode Resistance

Herbicide Tolerance

Cold Tolerance

Wood Properties

Sterility

Self-Incompatibility and Cross-Compatibility

Fruit Ripening

Rooting of Cuttings

Symbiosis

Other Traits

The Track Record

Crops Generally

Trees Specifically

Drawbacks and Difficulties

Applications to Forest Trees

Integration into Tree Breeding Programmes

Conclusions

7.    SOMACLONAL VARIATION

Principles and Experimental Achievements

Disease Resistance

Herbicide Tolerance

Tolerance to Salt and Other Stresses

Application to Forest Tree Improvement

8.    PROTOPLAST FUSION

Principles and Experimental Achievements

Application to Forest Tree Improvement

9.    HAPLOID CULTURES

Principles and Experimental Achievements

Application to Forest Tree Improvement

10.   IN VITRO EMBRYO RESCUE

Principles and Experimental Achievements

Application to Forest Tree Improvement

11.   MICROPROPAGATION

Principles and Experimental Achievements

Axillary Budding

Adventitous Budding

Somatic Embryogenesis

Application to Forest Tree Improvement

Cost of Planting Stock

Clonal Fidelity

Field Performance of Propagules

Multiplication Rate

Applicability to Adult Material

Compatibility with Culture Storage

Proportion of genetypes Responding

Conclusions

12.   IN VITRO CONTROL OF THE MATURATION STATE

Principles and Experimental Achievements

Application to Forest Tree Improvement

The Role of Juvenility in Clonal Forestry

The Effect of Generation Interval on Genetic Gain

Conclusions

13.   GENERAL CONCLUSIONS

The Status of Tree Improvement

Biotechnologies and their Applications to Tree Improvement

Cryopreservation and In Vitro Storage

Molecular Markers

In Vitro Selection

Genetic Engineering

Protoplast Fusion

Gametophyte Cultures

In Vitro Embryo Rescue

Micropropagation

In Vitro Control of the Maturation State

Synthesis

Biotechnology and Tree Improvement in Developing Countries

REFERENCES

GLOSSARY

ACRONYMS

Cover photo:
Linkage map of the Pinus elliottii x P. caribaea F1 hybrid.
Photo: G. Dale