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Sucrose, photosynthetic photon flux density, & CO2 concentration affect growth & development of micropropagated mountain ash

XV World Forestry Congress, 2-6 May 2022










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    High-frequency regeneration of plants in vitro from seedling-derived apical bud explants in Tilia mandshurica
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Tilia species have taken hold as a crop of interest for both the honey and timber industries, underscoring a need for the development of efficient methods for large-scale propagation. In Tilia species such as T. mandshurica, propagation through seeds is difficult because of a low germination rate (approximately 10-16%) and an extremely hard seed coating that can delay germination for up to two years. Further, cutting propagation is not efficient due to the resulting poor development of root systems. To overcome these difficulties, in vitro culture techniques for plant regeneration have become attractive methods for the propagation of plants. Generally, plant regeneration systems based on apical and axillary bud culture are the most effective method of in vitro propagation
    This work was to develop an efficient method for shoot induction and plant regeneration of seedling-derived apical bud explant in Tilia mandshurica. The highest rate (82.2%) of shoot induction was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on MS medium containing 1.0 mg/L BAP. However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. For rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5-1.0 mg/L IBA or 3.0 mg/L NAA. In the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) or root length (18.5 cm). This work showed that a low concentration (0.5 mg/L) of IBA is appropriate for rooting. Keywords: Biodiversity conservation ID: 3622528
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    Efficiency of total volatile organic compound of climbing plants Ardisia pusilla and Ficus pumila
    XV World Forestry Congress, 2-6 May 2022
    2022
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    This study was conducted to analyze removal efficiency of total volatile organic and physiological responses of climbing native plants Ardisia pusilla and Ficus pumila. The Hedera helix as a control, plants were investigated the total volatile organic compounds and their corresponding physiological responses with the maximum quantum yield of PSII (Fv/Fm)chlorophyll fluorescence, the vegetation index (the normalized difference vegetation index, NDVI; the simple ratio, SR; the carotenoid reflectance index 1, 2; CRI 1, 2) and physiological activity (the flavonoids and the phenolics). As a result, the removal amount of native plants tended to be lager than Hedera helix (2.973mgm-3h-1m-2 leaf area) and Ficus pumila (4.481mgm-3h-1m-2 leaf area) had more removal than Ardisia pusilla (3.786 mgm-3h-1m-2 leaf area), significantly. The NDVI, SR, and CRI 1, 2 of Ficus pumila and Hedera helix decreased after 2 hours, but there was no difference before and after with pollutants in Ardisia pusilla. In addition, only Ardisia pusilla had the normal range of 0.78 to 0.84 with Fv/Fm. Through this result, it is judged Ardisia pusilla is normally metabolized in plants without negative effects when removing indoor pollutants. Additionally, Ardisia pusilla did not change about phenolic, but the flavonoids contents increased after 2 hours. The physiological activity of Ficus pumila increased from 4 hours and then dereased again at 6 hours. Therefore, the removal of total volatile organic compounds was effective in the Ficus pumila, Ardisia pusilla, Hedera helix order, but in the results of stress and physiological activity of plants after air purification, Ardisia pusilla maintained a normal range than other plants and increased antioxidants. It is considered to be highly useful as an air purification plant due to its low damage. Keywords: Human health and well-being, Climate change, Covid-19, Research, Landscape management ID: 3622900
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    Germination characteristics and storage behavior of Codonopsis pilosula Nannf. seeds
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Codonopsis pilosula Nannf. is classified as an endangered species (VU) in Korea. Ex situ conservation through long-term storage of seeds is, in principle, possible for a significant proportion of plants. However, it is not known whether these seeds are orthodox; that is, if they can survive under long-term storage conditions (-20 °C, 40 % relative humidity (RH) or less) without being damaged. We determined the germination characteristics and storage behavior of C. pilosula. The seeds were collected from plants growing at the Baekdudaegan Arboretum on September 17, 2020. Immediately after collection, the equilibrium relative humidity (eRH) of the seeds was measured. To determine the germination characteristics of the seeds, a germination test was conducted at temperatures of 15, 20, and 25 °C (12/12 h) with 100 and 250 ppm GA3. To determine the storage behavior, their moisture content was measured under five conditions: 15 °C and RH at 15, 20, 30, 40, and 50 %, at 250 ppm GA3. Vitality was calculated as the ratio of the number of germinated seeds and the survived seeds of the unsprouted through the TZ test. Viability tests were conducted in the order of X-ray, germination test, and TZ test. The average germination percentage (GP) of untreated seeds was below 11 ± 3 %. The highest GP of seeds following GA3 treatment was 90 ± 0.70 %. The eRH was 45 % after collection. The initial seed viability percentage was 90.3 ± 2 %. The highest viability percentage (VP) was identified at 91 ± 0.25 % in equilibrium with 20 % (eRH) at 15 °C with a moisture content of 3.23 % Fresh weight. The results of our study on the behavior of C. pilosula seeds indicate that orthodox seeds did not reduce in viability even when dried in equilibrium with 20 % RH; thus, these seeds can be kept under long-term storage conditions. Keywords: Biodiversity conservation, Climate change, Agriculture ID: 3622450

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