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DocumentHigh-frequency regeneration of plants in vitro from seedling-derived apical bud explants in Tilia mandshurica
XV World Forestry Congress, 2-6 May 2022
2022Also available in:
No results found.Tilia species have taken hold as a crop of interest for both the honey and timber industries, underscoring a need for the development of efficient methods for large-scale propagation. In Tilia species such as T. mandshurica, propagation through seeds is difficult because of a low germination rate (approximately 10-16%) and an extremely hard seed coating that can delay germination for up to two years. Further, cutting propagation is not efficient due to the resulting poor development of root systems. To overcome these difficulties, in vitro culture techniques for plant regeneration have become attractive methods for the propagation of plants. Generally, plant regeneration systems based on apical and axillary bud culture are the most effective method of in vitro propagation
This work was to develop an efficient method for shoot induction and plant regeneration of seedling-derived apical bud explant in Tilia mandshurica. The highest rate (82.2%) of shoot induction was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on MS medium containing 1.0 mg/L BAP. However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. For rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5-1.0 mg/L IBA or 3.0 mg/L NAA. In the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) or root length (18.5 cm). This work showed that a low concentration (0.5 mg/L) of IBA is appropriate for rooting. Keywords: Biodiversity conservation ID: 3622528 -
DocumentSmell the disease - Developing rapid, high-throughput and non-destructive screening methods for early detection of alien invasive forest pathogens and pests featuring next-generation technologies
XV World Forestry Congress, 2-6 May 2022
2022Also available in:
No results found.Global forests are increasingly threatened by alien invasive pathogens and pests. The magnitude of this threat is expected to further increase in the future, due to the warmer climate and more extensive global transports and trade of plants. Pests and pathogens are often introduced to new areas by trade with ornamental plants as intermediate hosts, and there is a great need to modernize the tools for detection of alien species in imported plants and in monitoring of those that are already established in our forests. To achieve this goal, research in forest pathology is focused on combining recent technological advances in robotics, next generation sequencing, and mass spectroscopic methods with knowledge about the specific metabolic responses in the pests and pathogens and the trees that they infest. Gas Chromatography (GC) Analysis of Volatile Organic Compounds (VOCs) adsorbed on Solid Phase Micro-Extraction (SPME) fibers is one promising method with potential for high-throughput detection of larger plant shipments. By the establishment of a library of chemical fingerprints characterizing specific pests and pathogens, one could non-destructively scan a large number of plants in ports or nurseries to eliminate presence of disease. The species-specific combination of VOCs can be utilized to prevent introduction of harmful pests and pathogens to new markets. One pathogen considered as a quarantine species and a serious threat on-the-horizon for coniferous forests is Pine Pitch Canker (PPC), a fungal pathogen affecting a variety of pine species with devastating economical and biological consequences, especially if it were to be established in a country like Sweden where about 38% of the standing forest volume consist of pine. Pathogens like this one are already introduced in several European countries, and need to be monitored and identified early to prevent further forest damage – a challenge that Forest pathologists have accepted. Keywords: Climate change, Sustainable forest management, Research, Monitoring and data collection, Deforestation and forest degradation ID: 3499048 -
Poster, bannerScreen background for internal communications (FAO intranet, screen at the FAO Building A entrance, screen at external security pavillion to welcome external participants during Bioenergy Week, on the screens outside of the meeting room/s where the event takes place) 2024
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