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DocumentWood transcriptome profiling identifies critical pathway genes of secondary wall biosynthesis and novel regulators for vascular cambium development in populus
XV World Forestry Congress, 2-6 May 2022
2022Also available in:
No results found.Wood, the most abundant biomass on Earth, is composed of secondary xylem differentiated from vascular cambium. However, the underlying molecular mechanisms of wood formation remain largely unclear. To gain insight into wood formation, we performed a series of wood-forming tissue-specific transcriptome analyses from a hybrid poplar (Populus alba × P. glandulosa, clone BH) using RNA-seq. Together with shoot apex and leaf tissue, cambium and xylem tissues were isolated from vertical stem segments representing a gradient of secondary growth developmental stages (i.e., immature, intermediate, and mature stem). In a comparative transcriptome analysis of the ‘developing xylem’ and ‘leaf’ tissue, we could identify critical players catalyzing each biosynthetic step of secondary wall components (e.g., cellulose, xylan, and lignin). Several candidate genes involved in the initiation of vascular cambium formation were found via a co-expression network analysis using abundantly expressed genes in the ‘intermediate stem-derived cambium’ tissue. We found that transgenic Arabidopsis plants overexpressing the PtrHAM4-1, a GRAS family transcription factor, resulted in a significant increase of vascular cambium development. This phenotype was successfully reproduced in the transgenic poplars overexpressing the PtrHAM4-1. Taken together, our results may serve as a springboard for further research to unravel the molecular mechanism of wood formation, one of the most important biological processes on this planet. Keywords: Genetic resources, Research ID: 3622616 -
DocumentWood transcriptome analysis of Pinus densiflora identifies genes critical for secondary cell wall formation and NAC transcription factors involved in tracheid formation
XV World Forestry Congress, 2-6 May 2022
2022Also available in:
No results found.Although conifers have significant ecological and economic value, information on transcriptional regulation of wood formation in conifers is still limited. Here, to gain insight into secondary cell wall (SCW) biosynthesis and tracheid formation in conifers, we performed wood tissue-specific transcriptome analyses of Pinus densiflora (Korean red pine) using RNA sequencing. In addition, to obtain full-length transcriptome information, PacBio single molecule real-time (SMRT) iso-sequencing was carried out using RNAs from 28 tissues of P. densiflora. Subsequent comparative tissue-specific transcriptome analysis successfully pinpointed critical genes encoding key proteins involved in biosynthesis of the major secondary wall components (cellulose, galactoglucomannan, xylan, and lignin). Furthermore, we predicted a total of 62 NAC (NAM, ATAF1/2 and CUC2) family transcription factor members and identified seven PdeNAC genes preferentially expressed in developing xylem tissues in P. densiflora. Protoplast-based transcriptional activation analysis found that four PdeNAC genes, homologous to VND, NST and SND/ANAC075, upregulated GUS activity driven by an SCW-specific cellulose synthase promoter. Consistently, transient overexpression of the four PdeNACs induced xylem vessel cell-like SCW deposition in both tobacco (Nicotiana benthamiana) and Arabidopsis leaves. Taken together, our data provide a foundation for further research to unravel transcriptional regulation of wood formation in conifers, especially SCW formation and tracheid differentiation. Keywords: Research, Genetic resources ID: 3622610 -
Brochure, flyer, fact-sheetFood loss analyses to identify critical loss points, main causes of losses and to recommend solutions to reduce post-harvest losses
Grain supply chains in Burkina Faso, the Democratic Republic of the Congo and the Republic of Uganda - Technical brief
2021Also available in:
No results found.The UN Rome-based agencies joint project aimed to improve smallholder food security and incomes in food deficit areas by reducing food losses. The Project contributes to the African Union Malabo Declaration (AUC, 2014) in which Member States committed to halve the levels of post-harvest losses by 2025. It also contributes to the achievement of the Sustainable Development Goal (SDG) 12 on ensuring sustainable consumption and production patterns, specifically to target 12.3 that aims to halve food waste and reduce post-harvest losses by 2030. This technical brief presents the FAO methodology used to carry out studies on food loss analyses and the major findings and recommendations formulated to reduce post-harvest losses in the selected food supply chains in Burkina Faso (maize, sorghum, and cowpea), the Democratic Republic of the Congo (maize and rice) and the Republic of Uganda (maize, beans and sunflower), in the framework of the United Nations Rome-based Agencies’ joint project.
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