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The propagation of Balanites aegyptiaca (L.) Del.: Strategies

XV World Forestry Congress, 2-6 May 2022










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    Propagation and growth from seeds and root segment cuttings of fruit tree-plus Diospyros mespiliformis (Ebenaceae) in Benin West Africa
    XV World Forestry Congress, 2-6 May 2022
    2022
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    The aim of the present experiment was to assess effectiveness of selected propagation methods, early nursery and field performance of D. mespiliformis (Ebenaceae). For instance, effects of seed pretreatment/root segment cuttings diameter, provenance and soil types on germination and growth vigor of the species was investigated using 144 seeds/root segment; laid in 500 g of three different soil types and soaked/planted in polyethylene bags replicated four times in three treatments and laid in 4×3×3 factorial Completely Randomized Design. Emergence of radicle (days), Seedlings/Shoot Height (cm), number of leaves, and Percentage survival were recorded two weekly for a period of 7 months. Time to event seed germination/shooting data analysis, ANOVA two ways and Generalized Mixed effects Model were used to assess effects of origin, pre treatment/diameter and soil types on germination, shooting and growth of Diospyros mespiliformis. Results indicated that propagation of the species can best be done through seeds and root cuttings. The essay revealed that seeds from woodland, savannah and parkland, sowed in compost and clay and pre treated (boiled water, tegument removed) had the highest germination percentage (69.9-86.67 %), while seeds in the control (no treatment, Inundated Forest and sandy) had very low germination of 17-35 %. No seed treatment showed deficiency in the survival and germination of the seeds of Diospyros mespiliformis, while borehole water aided the survival and bring back early (rapid) germination. Regarding RSC, leafy shoots formed percentage varied from 0 % in river sand to 64.6% in clay and compost substrate after 12 weeks. Results reveal also that among the three classes of diameter, shooting height decreases from clay to sandy while the same variable move in inverse order regarding RSC origin. Keywords: Adaptive and integrated management, Biodiversity conservation, Genetic resources, Research, Sustainable forest management ID: 3477094
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    High-frequency regeneration of plants in vitro from seedling-derived apical bud explants in Tilia mandshurica
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Tilia species have taken hold as a crop of interest for both the honey and timber industries, underscoring a need for the development of efficient methods for large-scale propagation. In Tilia species such as T. mandshurica, propagation through seeds is difficult because of a low germination rate (approximately 10-16%) and an extremely hard seed coating that can delay germination for up to two years. Further, cutting propagation is not efficient due to the resulting poor development of root systems. To overcome these difficulties, in vitro culture techniques for plant regeneration have become attractive methods for the propagation of plants. Generally, plant regeneration systems based on apical and axillary bud culture are the most effective method of in vitro propagation
    This work was to develop an efficient method for shoot induction and plant regeneration of seedling-derived apical bud explant in Tilia mandshurica. The highest rate (82.2%) of shoot induction was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on MS medium containing 1.0 mg/L BAP. However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. For rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5-1.0 mg/L IBA or 3.0 mg/L NAA. In the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) or root length (18.5 cm). This work showed that a low concentration (0.5 mg/L) of IBA is appropriate for rooting. Keywords: Biodiversity conservation ID: 3622528
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    Mass propagation of a Dendrocalamus asper through seedling macroproliferation for large-scale plantation programs
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Dendrocalamus asper is a thick walled edible bamboo species with diversified industrial uses. Vegetative propagation trough culm, rhizome or branch cutting is difficult due to inadequate numbers. Tissue culture requires sophisticated labs, expensive procedure and impractical in many areas of the world. Upon availability, seed germination and seedling macroproliferation could be very simple, inexpensive and advantageous for bamboo propagation which start the physiological age from zero. Current study was therefore carried out over a period of two year from March 2017 to February 2019 to explore the regeneration potentials of D. asper through seed germination and seedling macro-proliferation. Intact or deglumed seeds were soaked in water for 0h, 24h or 48h and sown in pots filled with soil and cow dung under nursery shed (T1) and intact seeds soaked in water mixed with fungicide for 24h were sown in perforated plastic tray filled with soil and cow dung in greenhouse condition (T2) for germination. The fastest germination (7 days) and maximum germination percent (60.3) was in deglumed seeds soaked in water for 24h followed by 40.27% in the same seeds soaked for 48h in T1. Seedlings developed through various pre-sowing treatments were with 4-9 tillers each and macroproliferated six months after germination and grown for another six month for second cycle proliferation. Through macroproliferation, average number of propagules produced were 4 and 8.2 in first cycle and second cycle respectively. Thus 32.8 number of planting materials were produced from a single seedling within one and half year period of time which could be 289.8 through third cycle proliferation at the end of two years. Survival rate of macroproliferated propagules was 86.6% one month after transferring them in the sun. Propagule were ready for third cycle macroproloferation (with 9.06 number of tillers again in each propagule) six months or for planting nine months after the second cycle of macroproliferation. Findings of this study could be a basis for further study and for re-greening the earth for better resilience to climate change adaptations. Keywords: Edible bamboo, deglume seeds, Dendrocalamus asper, seed germination, seedling macroproliferation. ID: 3476549

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