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Induced Spawning in Catfish Clarius macrocephalus by Pituitary Hormone Injection





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    Induced spawning and larval rearing of seabass (Lates calcarifer Bloch) in captivity 1989
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    Seabass (Lates calcarifer) were successful induced to spawn in captivity by hormonal induction. The hormones used for the trials were HCG + Synthetic hormones (Puberogen). The dosages used were 250 IU HCG + 50 RU of Puberogen per kg of fish for first injection and 500 IU HCG + 100 RU of Puberogen per kg of fish for the second injection at an interval of 24 hrs. The fish responded well to the hormones and dosage given. The fish spawned naturally in a 8-ton fibreglass tank within 10 hrs after the second injection. The larvae were reared in a 1.7 tons fiberglass circular tank at an initial stocking density of 60–100 larvae per litre. Feed used were Tetraselmis chuii + Brachionus + Artemia nauplii + subadult Artemia + minced fish. The third batch produced 600 000 of 30-day fry. The average survival rate from yolkfish to 30 days old fry was about 45 percent. The results suggested that inducing the fish to spawn during off spawning season by hormones manipulation might result in high per centage of unfertilized eggs and abnormal fry. As other marine fish, the seabass fry required a diet containing high level of highly unsaturated fatty acid (HUFA) to obtain fast growth and high survival rate.

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