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Book (series)DNA-based molecular diagnostic techniques: research needs for standardization and validation of the detection of aquatic animal pathogens and diseases.
Report and proceedings of the Expert Workshop on DNA-based Molecular Diagnostic Techniques: Research Needs for Standardization and Validation of the Detection of Aquatic Animal Pathogens and Diseases. Bangkok, Thailand, 7-9 February 1999.
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No results found.In efforts to limit trans-boundary movement of pathogens and reduce the economic and socioeconomic impact of disease in aquaculture, there is considerable scope for more effective use of DNA-based methods of pathogen detection. These technologies offer rapid results with potentially high sensitivity and specificity, at relatively low cost. Recognition of these advantages has led to rapid adoption of available DNA-based tests, particularly in shrimp culture for which histological procedures lack specificity and culture-based methods have not been possible. However, few if any of the available tests have been assessed appropriately against other diagnostic methods or standardized and validated for specified applications. In fish and shrimp, type or strain specificity of most tests for pathogens in the Asian region is poorly understood and, in molluscs, there is little information on the significant pathogens and few tests of any kind have been developed. Furthermore, tests presently avai lable are frequently conducted by technicians who may not be sufficiently aware of the need for stringent test protocols or the meaning and limitations of the data generated. Implementation of standardized practices that produce reliable, useful and comparable data will require a significant investment in research, training and infrastructure development. Effective implementation will also be assisted by enhanced communication between aquatic animal health practitioners in the region and scienti sts with expertise in molecular diagnostic technologies. This review recommends development by FAO/NACA of 2 programs of managed cooperative research to assist more effective use of DNA-based detection tests. Program A should focus on improving the knowledge base by identification of new and emerging pathogens, relating pathogens in the region to those described elsewhere, and defining the extent of genetic variation between related pathogens in the region. Program B should draw on information currently available or obtained from Program A to develop suitably specific DNA-based diagnostic methods and to evaluate and validate the methods for disease diagnosis and pathogen screening programs. To increase the availability of scientists and technicians with skills in pathology and molecular diagnostic technologies, the review also recommends development of FAO/NACA-sponsored training programs for staff from key laboratories in the region. Training priorities should be in: i) the use of standard histopathological methods for health screening of fish and molluscs; and ii) the use of standard DNA-based methods for pathogen detection including sample collection, application of test protocols and the analysis and interpretation of test results. Because of the urgency of disease problems and the availability of suitable tests, training in DNA-based methods should focus initially on detection of shrimp pathogens. The review also recommends the development of a laboratory accredita tion program in order to achieve standardization of sampling methods and test procedures. The establishment of reference laboratories will assist accreditation for each of the major pathogens. Laboratory accreditation and training programs should complement the activities of OIE in obtaining internationally agreed test standards for molecular diagnostic technologies. -
Book (stand-alone)The living marine resources of the Western Central Atlantic. Volume 1. Introduction, molluscs, crustaceans, hagfishes, sharks, batoid fishes and chimaeras. 2002
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No results found.This is the first of a three volumes field guide that covers the species of interest to fisheries of the major marine resources groups exploited in the Western Central Atlantic. The area of coverage includes FAO Fishing Area 31. The marine resources groups included in this volume are the bivalves, gastropods, cephalopods stomatopods, shrimps, lobsters, crabs, hagfishes, sharks, batoid fishes and chimaeras. The introductory chapter outlines the environmental, ecological and biogeographical factor s influencing the marine biota and the basic components of the fisheries in the Western Central Atlantic. See also other volumes related to this series: -
Book (series)Report of the Technical Workshop on the Development of a Strategy for Improving Biosecurity (Aquatic Animal Health) in the Subregional Countries of the Mozambique Channel (Madagascar, Mozambique and the United Republic of Tanzania). 2015
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No results found.The incursion of the white spot syndrome virus (WSSV), the most serious pathogen of cultured shrimp, into Mozambique and Madagascar prompted national governments and international agencies such as the World Bank and FAO to develop long-term policy actions through a strategy that will improve aquatic biosecurity governance in the three countries (Madagascar, Mozambique and the United Republic of Tanzania) of the Mozambique Channel, so that the risks of diseases affecting shrimp aquaculture can be reduced. The Technical Workshop on the Development of a Strategy for Improving Biosecurity (Aquatic Animal Health) in the Subregional Countries of the Mozambique Channel (Madagascar, Mozambique and the United Republic of Tanzania), held from 2 to 4 April 2013 in Maputo, Mozambique, was organized and facilitated by FAO with financial support from the World Bank and hosted by the National Institute for Development of Aquaculture and the Institute for Fish Inspection of Mozambique.
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