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Book (series)DNA-based molecular diagnostic techniques: research needs for standardization and validation of the detection of aquatic animal pathogens and diseases.
Report and proceedings of the Expert Workshop on DNA-based Molecular Diagnostic Techniques: Research Needs for Standardization and Validation of the Detection of Aquatic Animal Pathogens and Diseases. Bangkok, Thailand, 7-9 February 1999.
2000Also available in:
No results found.In efforts to limit trans-boundary movement of pathogens and reduce the economic and socioeconomic impact of disease in aquaculture, there is considerable scope for more effective use of DNA-based methods of pathogen detection. These technologies offer rapid results with potentially high sensitivity and specificity, at relatively low cost. Recognition of these advantages has led to rapid adoption of available DNA-based tests, particularly in shrimp culture for which histological procedures lack specificity and culture-based methods have not been possible. However, few if any of the available tests have been assessed appropriately against other diagnostic methods or standardized and validated for specified applications. In fish and shrimp, type or strain specificity of most tests for pathogens in the Asian region is poorly understood and, in molluscs, there is little information on the significant pathogens and few tests of any kind have been developed. Furthermore, tests presently avai lable are frequently conducted by technicians who may not be sufficiently aware of the need for stringent test protocols or the meaning and limitations of the data generated. Implementation of standardized practices that produce reliable, useful and comparable data will require a significant investment in research, training and infrastructure development. Effective implementation will also be assisted by enhanced communication between aquatic animal health practitioners in the region and scienti sts with expertise in molecular diagnostic technologies. This review recommends development by FAO/NACA of 2 programs of managed cooperative research to assist more effective use of DNA-based detection tests. Program A should focus on improving the knowledge base by identification of new and emerging pathogens, relating pathogens in the region to those described elsewhere, and defining the extent of genetic variation between related pathogens in the region. Program B should draw on information currently available or obtained from Program A to develop suitably specific DNA-based diagnostic methods and to evaluate and validate the methods for disease diagnosis and pathogen screening programs. To increase the availability of scientists and technicians with skills in pathology and molecular diagnostic technologies, the review also recommends development of FAO/NACA-sponsored training programs for staff from key laboratories in the region. Training priorities should be in: i) the use of standard histopathological methods for health screening of fish and molluscs; and ii) the use of standard DNA-based methods for pathogen detection including sample collection, application of test protocols and the analysis and interpretation of test results. Because of the urgency of disease problems and the availability of suitable tests, training in DNA-based methods should focus initially on detection of shrimp pathogens. The review also recommends the development of a laboratory accredita tion program in order to achieve standardization of sampling methods and test procedures. The establishment of reference laboratories will assist accreditation for each of the major pathogens. Laboratory accreditation and training programs should complement the activities of OIE in obtaining internationally agreed test standards for molecular diagnostic technologies. -
Book (series)Exposure of humans or animals to SARS-CoV-2 from wild, livestock, companion and aquatic animals
Qualitative exposure assessment
2020Also available in:
No results found.Understanding the risk of exposure of humans or animals to SARS-CoV-2 from animals and their products is essential for containing virus spread, prioritizing research, protecting food systems, and informing national One Health investigations and mitigation measures. This Qualitative Exposure Assessment provides a comprehensive review of available scientific evidence and assessment of exposure risk from different wild or domestic animal species. Results can inform country-level risk assessment and provide the evidence base for targeted SARS-CoV-2 investigations in animals and mitigation options. This publication provides: I. assessment of the risk of human or animal exposure to SARS-CoV-2 through contact with, handling or consumption of wild, domestic and aquatic animal species or their products; II. identification of current knowledge gaps regarding the zoonotic origin or animal-human spillover of SARS-CoV-2 and recommendations on priority studies; III. summary of available evidence for SARS-CoV-2 susceptibility of different animal species; IV. evidence-based recommendations on how to prioritize animal species for targeted field investigations or research studies; V. recommendations for targeted One Health investigations and epidemiological, laboratory, anthropological or seasonality studies to fill critical knowledge gaps evidenced by this exposure assessment. -
Book (stand-alone)Report of the Introductory Training Course on Risk Analysis for Movements of Live Aquatic Animals for RECOFI Members and the Round-table Meeting on RECOFI Regional Aquatic Biosecurity, Muscat, Sultanate of Oman, 1-5 November 2015 2017
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No results found.The Introductory Training Course on Risk Analysis for Movements of Live Aquatic Animals for RECOFI Members and the Round-table Meeting on RECOFI Regional Aquatic Biosecurity were held from 1 to 4 November and on 5 November, respectively, in Muscat, Sultanate of Oman. Both activities were hosted by the Ministry of Agriculture and Fisheries Wealth (MAFW), Sultanate of Oman.
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