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DocumentCharacterization of formate dehydrogenase from Trametes versicor for formate production from CO2 gas
XV World Forestry Congress, 2-6 May 2022
2022Also available in:
No results found.Enzymatic CO2 reduction has been reported as a promising approach to greenhouse gas fixation. There are few biocatalysts capable of biological CO2 fixation, e.g. pyruvate decarboxylase, carbonic anhydrase, and formate dehydrogenase. Formate dehydrogenase(FDH) can reduce CO2 to formate without organic chemicals, and formate can be sequentially reduced to formaldehyde and methanol by coupling aldehyde dehydrogenase and alcohol dehydrogenase reactions. Therefore, FDH has been widely adopted inCO2 reducing reaction.
In this study, we researched CO2-reducing activities of FDH derived from wood rot fungi, Trametes versicolor. Microbial species, T. versicolor, were separated in National institute of Forest Science. The fungi were grown in a potato dextrose agar medium. For the transformation, the host, Shizosaccaromyces pombe of wild type, was obtained from Bioneer(Korea). Total RNA was extracted with Hybrid-R (GeneAll) from T. versicolor mycelium. cDNAs were synthezied by AccuPower (Bioneer) as recommended by the manufacturer. For sub-cloning, PCR of fragments (1.2 kb) were amplified with pairs of TvFDH ORF primers(Foword: 5’-ATGCTCGCCGGCATCT-3’, Reverse: 5’-TCACTTGCGCTGGCCA-3’). The amplified fragments were sub-cloned into a pCR2.1-TOPO (Invitrogen, Carlsbad, CA, USA) vector and the plasmid DNA was sequenced. The amplified fragments were digested by Not 1 and Bam 1 and then inserted into a pSLF272 vector (Bioneer, Korea). The recombinant plasmid was amplified in the Escherichia coli DH5α, and then extracted by mini prep kit. The recombinant plasmids were chemically (lithium acetate method) transformed into yeast S. pombe. Ligation products, transformants, were named as pSLF272-TvFDH. Selection of transformants were performed in the minimal medium, EMM. Expression and purification of the TvFDHs is being performed to obtain pure enzymes. Keywords: Climate change, Research, Genetic resources, Agriculture, Innovation ID: 3604933 -
Book (stand-alone)The convergence of food diets: Characterizing consumption patterns, food diversity, and the relationship to trade
Background paper for The State of Agricultural Commodity Markets (SOCO) 2020
2020Also available in:
No results found.Since the 1990s, technological advancements, growing incomes, increased trade, and urbanization have significantly impacted consumption patterns. Worldwide, there is growing evidence of some convergence of diets being facilitated by rapid changes in global food systems including the increasing market share held by supermarkets at all income levels. The formation of the World Trade Organization (WTO) and the emergence and rapid spread of the Internet have also played important roles in facilitating trade and increasing the variety of food available to consumers. Empirical evidence to examine these impacts has mostly been gathered at the household level and, at the global level, the focus has been on the effect of globalization on obesity and health. Using data from the periods 1994–1996 (WTO formation and emergence of the Internet) and 2015–2017 (rapid spread of the Internet), this paper analyses whether global diets are, in fact, converging. In the comparison of these two periods, the author finds that, as trade intensity increases for cereals, sugars, vegetable oils, and meat – which account for more than two-thirds of calories consumed – so does diversity of products consumed from within each group. The relationship between greater trade intensity and caloric consumption diversity is strongest for cereals, meat, and sugars. The author suggests that further research should undertake a disaggregated trade analysis in order to understand whether the increased food diversity is coming from imports of more diverse foods or other factors. -
Book (series)EAF-Nansen Programme expert workshop on ecosystem characterization
Rome, Italy 21–23 August 2018
2022Also available in:
No results found.As part of the EAF-Nansen Programme activities, a workshop was organized on ecosystem characterization at the FAO Headquarters in Rome from 21 to 23 August 2018. The workshop aimed at identifying relevant ecosystem characterization approaches for data limited areas, defining best-practice methods for ecosystem characterization in data limited regions and how these should be applied, suggesting how this knowledge can feed into decision-making at tactical and strategic levels, defining how uncertainty in the methods can be taken into account and communicated, and understanding existing challenges and ongoing efforts in targeted regions that may be of relevance to ecosystem characterization. The expected outputs of the workshop were a report describing best methods/approaches for ecosystem characterizations in data-limited regions and an action plan for future case studies in two regions.
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