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Standard operating procedure for boron determination in soil: Hot water extraction










FAO. 2024. Standard operating procedure for boron determination in soil: Hot water extraction. Rome.



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    Microbial biomass is considered as an estimation of soil biological activities and the capacity to mediate soil biochemical reactions. It is the most dynamic and labile of soil organic matter fractions, generally accounting for 1 to 5 percent of soil organic matter and very sensitive to soil management. The need to quantify soil microbiota has become increasingly relevant in current times as they are responsible for many different processes like the degradation of organic matter, the stability of aggregates and most of the nutrient cycling that occurs in soils. This standard operating procedure (SOP) focuses on the determination of soil microbial biomass using the chloroform fumigation-extraction method, which is applicable to both aerobic and anaerobic conditions over the whole range of soil pH, regardless of land use type. While chloroform fumigation also affects soil fauna, the carbon aliquot derived from these organisms is generally small (less than 5 percent) and can usually be disregarded.
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    Standard operating procedure for quasi total elements in soil by acid digestion, including heavy metals 2023
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    This method is applicable to the determination of trace element metals (MTE) as well as metalloids in soils by acid digestion using aqua regia.
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    Standard operating procedure for soil enzyme activities
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    This standard operating procedure (SOP) has been harmonized by the joint working group of the FAO's Global Soil Laboratory Network (GLOSOLAN) and the International Network on Soil Biodiversity (NETSOB). The protocol focuses on the determination of five soil enzyme activities: β-Glucosidases, Arylsulfatase, N-acetyl-β-Glucosaminidase, Dehydrogenase, and Phosphomonoesterases. After a general introduction on the importance of soil enzyme as key indicator for soil health and few remarks on the sampling strategies, the determination of each enzyme is presented separately with step-by-step instructions.

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