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Induced spawning and larval rearing of seabass (Lates calcarifer Bloch) in captivity







Chantarasri, S.; Santoso, H.; Hardoto; Yuwono, S.K. Mar 1989. Induced spawning and larval rearing of seabass (Lates calcarifer Bloch) in captivity. (np). 17 p.


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    The food and feeding of seabass Lates calcarifer, grouper Epinephelus tauvina and rabbitfish Siganus canaliculatus in floating net cages at the National Seafarming Development Centre, Lampung, Indonesia 1989
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    Four cage feeding trials involving a total of fifteen different dietary feeding regimes were conducted with seabass (Lates calcarifer), grouper (Epinephelus tauvina) and rabbitfish (Siganus canaliculatus) from fingerling to market size over a 100 to 156-day rearing period. Seabass fed frozen fish or a moist pellet displayed the best growth response, with fish (30/m3) growing from an initial weight of 8.9g and 8.5g to a final weight of 285.5g and 257.5g in 156 days, with a food conversion ratio o f 3.77 – 5.10 (dry matter basis 0.96 – 1.30) and 1.89 – 3.20 (dry matter basis 1.15 – 1.95), and with a survival of 93.3% and 93.3%, respectively. During the seabass trial the estimated feeding cost/kg fish produced was Rp 1458 – 1964 and Rp 1537 – 2584 for fish fed frozen fish and moist pellet, respectively. Grouper fed frozen fish and moist pellet also displayed the best growth response, with fish (30/m3) growing from an initial weight of 23.8g and 25.8g to a final weight of 471.7g and 388.7g in 156 days, with a food conversion ratio of 3.53 – 4.16 (dry matter basis 0.89 – 1.06) and 1.73 – 2.96 (dry matter basis 1.06 – 1.80), and with a survival of 90% and 98.3%, respectively. During the grouper trial the estimated feeding cost/kg fish produced was Rp 1342 – 1603 and Rp 1382 – 2396 for fish fed frozen fish and moist pellet, respectively. In the case of rabbitfish, the best growth was observed for fish fed a dry in-house crumble containing 31% crude protein; fish (15/m3) growing from an initial weight of 51.7g to a final weight of only 110.3g in 100 days, with a food conversion ratio of 3.48 (dry matter basis 3.08) and estimated feeding cost/kg fish produced of Rp 2349. However, all rabbitfish fed formulated rations displayed marked sexual activity by the end of the feeding trial (62 – 93% of the fish yielding milt or eggs on hand stripping).
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    Propagation of seabass, Lates calcarifer in captivity 1989
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    *
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    Induced spawning and larval rearing of grouper (Epinephelus salmoides Maxwell)
    Establishment of a Network of Aquaculture Centres in Asia
    1986
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    Broodfish of grouper (Epinephelus salmoides) were induced to spawn by hormonal induction. The hormones used for this experiment were HCG + pituitary gland (PG) and LRH-a. The results showed that at the dosages of 500 IU HCG + 3 mg of PG per kg of fish for first injection and 1,000 IU HCG + 3 mg of PG per kg of fish at the final injection at an interval of 24 hours, the treated fish spawned naturally in a spawning tank 12 hours after the final injection. At lower dosages of 500 IU HCG + 3 mg PG a t 12-hour intervals or 500 IU HCG + 3 mg PG at 24-hour intervals or using 10 mg LRH-a at 12-hour intervals, the eggs can be artificially fertilized only by stripping. * This paper was presented at the First Asian Fisheries Forum 24–30 May, 1986 Manila, Philippines. ** Present Address : FAO/UNDP Seafarming Development Project (INS/81/008), FAO, Jakarta, Indonesia. The larval rearing experiment ws conducted in a 250-liter fiberglass tank at the stocking density of 2,500 larvae per tank. Feed s used in this experiment were : (a) Isochrysis + sea urchin eggs; (b) Isochrysis + Brachionus; and (c) Tetraselmis + Brachionus. The experiment results showed that newly hatched larvae fed with Isochrysis mixed with sea urchin eggs and then switched to Brachionus on day 10 gave the best survival rate (9% at day 30).

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