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Characterization of formate dehydrogenase from Trametes versicor for formate production from CO2 gas

XV World Forestry Congress, 2-6 May 2022

Year of publication 2022

Place of publication Rome, Italy ;

Pages 1p.

Author Lee, S.-Y., Lee, S.-M., Park, M.-J., et al.

Publisher FAO ;

Product type Document

Synopsis (short abstract) Enzymatic CO2 reduction has been reported as a promising approach to greenhouse gas fixation. There are few biocatalysts capable of biological CO2 fixation, e.g. pyruvate decarboxylase, carbonic anhydrase, and formate dehydrogenase. Formate dehydrogenase(FDH) can reduce CO2 to formate without organic chemicals, and formate can be sequentially reduced to formaldehyde and methanol by coupling aldehyde dehydrogenase and alcohol dehydrogenase reactions. Therefore, FDH has been widely adopted inCO2 reducing reaction.
In this study, we researched CO2-reducing activities of FDH derived from wood rot fungi, Trametes versicolor. Microbial species, T. versicolor, were separated in National institute of Forest Science. The fungi were grown in a potato dextrose agar medium. For the transformation, the host, Shizosaccaromyces pombe of wild type, was obtained from Bioneer(Korea). Total RNA was extracted with Hybrid-R (GeneAll) from T. versicolor mycelium. cDNAs were synthezied by AccuPower (Bioneer) as recommended by the manufacturer. For sub-cloning, PCR of fragments (1.2 kb) were amplified with pairs of TvFDH ORF primers(Foword: 5’-ATGCTCGCCGGCATCT-3’, Reverse: 5’-TCACTTGCGCTGGCCA-3’). The amplified fragments were sub-cloned into a pCR2.1-TOPO (Invitrogen, Carlsbad, CA, USA) vector and the plasmid DNA was sequenced. The amplified fragments were digested by Not 1 and Bam 1 and then inserted into a pSLF272 vector (Bioneer, Korea). The recombinant plasmid was amplified in the Escherichia coli DH5α, and then extracted by mini prep kit. The recombinant plasmids were chemically (lithium acetate method) transformed into yeast S. pombe. Ligation products, transformants, were named as pSLF272-TvFDH. Selection of transformants were performed in the minimal medium, EMM. Expression and purification of the TvFDHs is being performed to obtain pure enzymes. Keywords: Climate change, Research, Genetic resources, Agriculture, Innovation ID: 3604933

Statutory body / Meeting title World Forestry Congress (WFC)

Language English

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