Thumbnail Image

Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica

Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82nd meeting 2016









Also available in:
No results found.

Related items

Showing items related by metadata.

  • Thumbnail Image
    Meeting
    Rosemary Extract (Tentative)
    Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82nd meeting 2016
    2016
    Also available in:
    No results found.

    Rosemary extract is obtained from ground dried leaves of Rosmarinus officinalis L using food-grade solvents, namely, acetone or ethanol. Solvent extraction is followed by filtration, solvent evaporation, drying and sieving to obtain a fine powder. Additional concentration and/or precipitation steps followed by deodorisation, decolourisation and standardisation using diluents and carriers of food grade quality maybe included to produce the final product. Rosemary extract is characterised by its c ontent of phenolic diterpenes, carnosic acid and carnosol, the principal antioxidative agents. Other antioxidant components present include triterpenes and triterpenic acids. Rosemary extract is identified by the total content of carnosol and carnosic acid as a ratio of reference volatile compounds which are responsible for flavour. The product of commerce can be standardized to a total carnosic acid and carnosol content up to 33%.
  • Thumbnail Image
    Meeting
    Lutein Esters from Tagetes Erecta
    Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82nd meeting 2016
    2016
    Also available in:
    No results found.

    Lutein esters from Tagetes erecta is obtained by solvent extraction of dried petals of Tagetes erecta L., further purification and subsequent removal of solvents under vacuum. Lutein diesters account for the major part and a smaller proportion of zeaxanthin diesters is also present. The esters contain saturated long chain fatty acids, such as myristic, palmitic and stearic acid in various proportions with palmitic acid being a major component. Waxes naturally occurring in the source material may also be present. Only the following solvents may be used in the production: methanol, ethanol, 2-propanol, hexane, acetone, methyl ethyl ketone and carbon dioxide. Usually food grade antioxidants are added to stabilize the product. Products of commerce are normally further formulated e.g. in order to standardize colour content or to obtain water soluble/dispersible products.
  • Thumbnail Image
    Meeting
    Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica
    Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82nd meeting 2016
    2016
    Also available in:
    No results found.

    Prepared at the 82nd JECFA (2016) and published in FAO JECFA Monograph 19 (2016), superseding specifications for Oxidized starch included in the specifications for Modified starches prepared at the 79th JECFA (2014), published in FAO JECFA Monographs 16 (2014). An ADI “not specified” was established at the 26th JECFA (1982). Rebaudioside A is a steviol glycoside composed predominantly of rebaudioside A with minor amounts of other steviol glycosides such as rebaudioside B, rebaudioside D, rebaudi oside M, and stevioside. Rebaudioside A is obtained from the fermentation of a non-toxigenic non-pathogenic strain of Yarrowia lipolytica that is genetically modified with heterologous genes from multiple donor organisms to overexpress steviol glycosides. After removal of the biomass by solid-liquid separation and heat treatment, the process involves concentration of the steviol glycosides (e.g. by resin adsorption), followed by purification of the rebaudioside A by crystallization and drying. I on exchange resins may be used in the purification process. The final product may be spray-dried.

Users also downloaded

Showing related downloaded files

No results found.