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Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica

Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82nd meeting 2016

Year of publication 2016

Pages 6p.

Publisher FAO ;

Product type Meeting

Category Meeting document

Edition 1

Synopsis (short abstract) Prepared at the 82nd JECFA (2016) and published in FAO JECFA Monograph 19 (2016), superseding specifications for Oxidized starch included in the specifications for Modified starches prepared at the 79th JECFA (2014), published in FAO JECFA Monographs 16 (2014). An ADI “not specified” was established at the 26th JECFA (1982). Rebaudioside A is a steviol glycoside composed predominantly of rebaudioside A with minor amounts of other steviol glycosides such as rebaudioside B, rebaudioside D, rebaudi oside M, and stevioside. Rebaudioside A is obtained from the fermentation of a non-toxigenic non-pathogenic strain of Yarrowia lipolytica that is genetically modified with heterologous genes from multiple donor organisms to overexpress steviol glycosides. After removal of the biomass by solid-liquid separation and heat treatment, the process involves concentration of the steviol glycosides (e.g. by resin adsorption), followed by purification of the rebaudioside A by crystallization and drying. I on exchange resins may be used in the purification process. The final product may be spray-dried.

Meeting Session Number Sess. 82

Language English

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    Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica
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    Steviol Glycosides from Stevia Rebaudiana Bertoni (Tentative)
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    Steviol glycosides consists of a mixture of compounds containing a steviol backbone conjugated to any number or combination of the principal sugar moieties in any of the orientations occurring in the leaves of Stevia rebaudiana Bertoni including, glucose, rhamnose, xylose, fructose, and deoxyglucose. The product is obtained from the leaves of Stevia rebaudiana Bertoni. The leaves are extracted with hot water and the aqueous extract is passed through an adsorption resin to trap and concentrate th e component steviol glycosides. The resin is washed with a solvent alcohol to release the glycosides and the product is recrystallized from methanol or aqueous ethanol. Ion exchange resins may be used in the purification process. The final product may be spray-dried.
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