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Standard operating procedure for soil respiration rate









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    Booklet
    Standard operating procedure for soil microbial biomass (carbon): chloroform fumigation-extraction method 2024
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    Microbial biomass is considered as an estimation of soil biological activities and the capacity to mediate soil biochemical reactions. It is the most dynamic and labile of soil organic matter fractions, generally accounting for 1 to 5 percent of soil organic matter and very sensitive to soil management. The need to quantify soil microbiota has become increasingly relevant in current times as they are responsible for many different processes like the degradation of organic matter, the stability of aggregates and most of the nutrient cycling that occurs in soils. This standard operating procedure (SOP) focuses on the determination of soil microbial biomass using the chloroform fumigation-extraction method, which is applicable to both aerobic and anaerobic conditions over the whole range of soil pH, regardless of land use type. While chloroform fumigation also affects soil fauna, the carbon aliquot derived from these organisms is generally small (less than 5 percent) and can usually be disregarded.
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    Standard operating procedure for soil available phosphorus - Olsen method 2021
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    This procedure is suitable for calcareous, alkaline, neutral, and slightly acidic soils containing CaPO4, since the calcium concentration in the solution is suppressed by the precipitation of CaCO3, increasing PO4 concentration in solution. Neutral and slightly acid soils (pH 6.0 to 7.0) may contain both Ca- and Al-phosphates. The NaHCO3 extractant can remove Ca-phosphates and phosphate adsorbed on the surface of calcium and magnesium carbonates along with Al-phosphates and is considered the most suitable extractant for these soils.
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    Standard operating procedure for soil moisture content by gravimetric method 2023
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    Soil moisture content is one of the fundamental properties used in making decisions related to soil management, such as land preparation, design of irrigation and drainage systems, construction, and risk management. Analytically, determination of air-dry moisture content (i.e. remaining moisture in soil, dried for laboratory analysis) is important, since it is used to express the results of other tests (e.g. carbon, nitrogen, phosphorus, etc.) on an oven-dry basis. Soil moisture content analyzed by the gravimetric method has low operational difficulty and can be used for multiple purposes.

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