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An improved technique for decapsulation and preservation of Artemia cysts (brine shrimp eggs) developed at the Chachoengsao Fisheries Station

Year of publication 1979

Pages p.6

Author Anand Tunsutapanich, (Ministry of Agriculture and Cooperatives, Chachoengsao (Thailand). National Freshwater Prawn Research and Training Centre);Fisheries and Aquaculture Management Division

Product type Project

Edition 1

Synopsis (short abstract) Decapsulation of brine shrimp eggs was originally carried out with sodium hydroxide and sodium hypochlorite at the Chacheongsao Fisheries Station. The cost of decapsulation by that technique was estimated at B300/kg. of brine shrimp eggs. A modified technique has been developed whereby decapsulation is conducted by using calcium oxide and calcium hypochlorite as stimulating agents. Promising results were achieved with the same degree of success as with the former technique. However, the latest t echnique is better because the cost of decapsulation can be reduced from B300/kg. to only B18/kg. of brine shrimp eggs.

Cite this content as:

Tunsutapanich, A. 1979. An improved technique for decapsulation and preservation of Artemia cysts (brine shrimp eggs) developed at the Chachoengsao Fisheries Station. 6 p.

Language English

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    This work paper is prepared in view of the wish of the Thai Department of Fisheries to inoculate salt ponds with Artemia salina to produce cysts. Artemia cysts are used to obtain live food in many aquacultural operations, but especially in the hatchery of the giant freshwater prawn, Macrobrachium rosenbergii. These inoculations, if successful, could encourage the Thai Government in the future to conduct experiments in large-scale cyst production. Indeed, up to this date, Thailand completely de pends on import of expensive Artemia cysts from abroad. The yearly expenditure in cysts by the Department of Fisheries for the national fisheries stations, is estimated to be more than US$50 000. The inoculation ponds considered are: (i) ponds in the National Fishery Station at Samut Sakhorn (still to be completed), and (ii) privately-owned salt ponds of salt farmers in the vicinity of the National Fishery Station at Chachoengsao. Although previously, several inoculations in other parts of t he world have proven to be successful, no written guides are yet available. Trial inoculations in Thailand being considered important, the necessity was felt to gather all available information on Artemia inoculations and cyst production into one working paper to serve as guide on improving the chances of a successful experiment.
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    Cysts production of Artemia salina in salt ponds in Thailand 1979
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    Techniques for culturing aquatic animals in freshwater, brackishwater and salt water have been improving rapidly. The feed for aquatic animals such as fish, shrimp, crab, etc. during their larval stages is therefore of vital importance. Up to now, the feeds used are planktonic plants and animals, e.g., diatoms, rotifiers, copepods, etc. which are reared in the hatchery or collected from natural breeding grounds. These practices are greatly handicapped by difficulties in obtaining sufficient quan tities of these foods. Artemia salina is one of the most important feeds. It has to be imported in large quantities from abroad. It is imported as dry Artemia cysts, preserved in vacuum packed tins in such a way that they will last for years. Subsequently, these cysts can be hatched whenever deemed necessary in the required quantity. However, the cost of imported cysts is extremely high. An experimental, research, training and development project for freshwater prawn culture at the Chacheongsa o Fisheries Station has been in operation for over 3 years. This project requires an enormous quantity of Artemia for its work. The Station has developed methods of utilizing Artemia to the fullest by conducting research in techniques for its preservation and establishing technical procedures for a new local Artemia rearing industry to replace the necessity of importing cysts. This report will deal briefly with the value of Artemia production and includes the techniques used and results of exp eriments on cyst production in Thai salt ponds.
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