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Genome-based Biotechnologies in Aquaculture

Year of publication 2017

Place of publication Rome, Italy ;

Pages 78 p.

Publisher FAO ;

Product type Document

Edition 1

Synopsis (short abstract) Genome technologies have been developed to study genome structure, organization, expression and function, and to select and modify genomes of interest to increase benefits to humans. Of these genome technologies, DNA marker technologies have been intensely used to map the genome to understand genome structure and organization. These DNA marker technologies include restriction fragment length polymorphism markers; mitochondrial DNA markers; DNA barcoding; random amplified polymorphic DNA markers; amplified fragment length polymorphism markers; microsatellite markers; single nucleotide polymorphism (SNP) markers; and restriction site-associated DNA sequencing markers (SNP markers per se). Although these marker systems have been used at various levels for various purposes, the microsatellite markers and SNP markers are currently the most important. Various genome mapping technologies have been developed, including both genetic mapping and physical mapping methods. Genetic mapping is based on recombination during meiosis, while physical mapping is based on fingerprints of DNA segments. Although several variations of physical mapping methods are available, such as radiation hybrid mapping and optical mapping, the most popular physical mapping method is the bacterial artificial chromosome based fingerprinting.

Language English

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