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Soaking seeds (seed priming) to improve crop yields










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    Propagation and growth from seeds and root segment cuttings of fruit tree-plus Diospyros mespiliformis (Ebenaceae) in Benin West Africa
    XV World Forestry Congress, 2-6 May 2022
    2022
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    The aim of the present experiment was to assess effectiveness of selected propagation methods, early nursery and field performance of D. mespiliformis (Ebenaceae). For instance, effects of seed pretreatment/root segment cuttings diameter, provenance and soil types on germination and growth vigor of the species was investigated using 144 seeds/root segment; laid in 500 g of three different soil types and soaked/planted in polyethylene bags replicated four times in three treatments and laid in 4×3×3 factorial Completely Randomized Design. Emergence of radicle (days), Seedlings/Shoot Height (cm), number of leaves, and Percentage survival were recorded two weekly for a period of 7 months. Time to event seed germination/shooting data analysis, ANOVA two ways and Generalized Mixed effects Model were used to assess effects of origin, pre treatment/diameter and soil types on germination, shooting and growth of Diospyros mespiliformis. Results indicated that propagation of the species can best be done through seeds and root cuttings. The essay revealed that seeds from woodland, savannah and parkland, sowed in compost and clay and pre treated (boiled water, tegument removed) had the highest germination percentage (69.9-86.67 %), while seeds in the control (no treatment, Inundated Forest and sandy) had very low germination of 17-35 %. No seed treatment showed deficiency in the survival and germination of the seeds of Diospyros mespiliformis, while borehole water aided the survival and bring back early (rapid) germination. Regarding RSC, leafy shoots formed percentage varied from 0 % in river sand to 64.6% in clay and compost substrate after 12 weeks. Results reveal also that among the three classes of diameter, shooting height decreases from clay to sandy while the same variable move in inverse order regarding RSC origin. Keywords: Adaptive and integrated management, Biodiversity conservation, Genetic resources, Research, Sustainable forest management ID: 3477094
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    Article
    Mass propagation of a Dendrocalamus asper through seedling macroproliferation for large-scale plantation programs
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Dendrocalamus asper is a thick walled edible bamboo species with diversified industrial uses. Vegetative propagation trough culm, rhizome or branch cutting is difficult due to inadequate numbers. Tissue culture requires sophisticated labs, expensive procedure and impractical in many areas of the world. Upon availability, seed germination and seedling macroproliferation could be very simple, inexpensive and advantageous for bamboo propagation which start the physiological age from zero. Current study was therefore carried out over a period of two year from March 2017 to February 2019 to explore the regeneration potentials of D. asper through seed germination and seedling macro-proliferation. Intact or deglumed seeds were soaked in water for 0h, 24h or 48h and sown in pots filled with soil and cow dung under nursery shed (T1) and intact seeds soaked in water mixed with fungicide for 24h were sown in perforated plastic tray filled with soil and cow dung in greenhouse condition (T2) for germination. The fastest germination (7 days) and maximum germination percent (60.3) was in deglumed seeds soaked in water for 24h followed by 40.27% in the same seeds soaked for 48h in T1. Seedlings developed through various pre-sowing treatments were with 4-9 tillers each and macroproliferated six months after germination and grown for another six month for second cycle proliferation. Through macroproliferation, average number of propagules produced were 4 and 8.2 in first cycle and second cycle respectively. Thus 32.8 number of planting materials were produced from a single seedling within one and half year period of time which could be 289.8 through third cycle proliferation at the end of two years. Survival rate of macroproliferated propagules was 86.6% one month after transferring them in the sun. Propagule were ready for third cycle macroproloferation (with 9.06 number of tillers again in each propagule) six months or for planting nine months after the second cycle of macroproliferation. Findings of this study could be a basis for further study and for re-greening the earth for better resilience to climate change adaptations. Keywords: Edible bamboo, deglume seeds, Dendrocalamus asper, seed germination, seedling macroproliferation. ID: 3476549
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    Book (stand-alone)
    Guidelines for the establishment and management of seed testing laboratories
    Joint ISTA and FAO Handbook
    2023
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    This Joint ISTA and FAO Handbook provides comprehensive guidelines for the setting up and managing of seed testing laboratories, including those that cater to small- and medium-scale seed enterprises and farmers’ cooperatives, which operate in low input production systems. The adherence to the guidelines by seed testing laboratories results in the use of uniform procedures across the board thereby facilitating inter-laboratory transferability of data. This harmonization enhances confidence in seed quality assurance mechanisms. The publication is an update of a previous one that was also jointly published by both organizations in 1969 and revised in 1979 and 1983. Its utility is enhanced by a set of new up-to-date information that builds upon existing data. The Handbook provides guidance on the range of seed testing equipment, procedures, and management systems that have become available in the several decades since the previous editions. Seed testing is a critical component of the seed value chain and is essential for the production of quality seeds, i.e. seeds that are alive, can germinate and produce vigorous seedlings; are healthy and come from lots that meet set thresholds for genetic and analytical purity. The Handbook covers all aspects of seed testing, from laboratory design and equipment selection to sample preparation, testing methods, and data analysis. Importantly, the publication also addresses the challenges of testing seeds in low input systems and provides guidance on how to adapt procedures to these settings. Therefore, it serves as a reference material and training resource for everyone involved in seed quality assurance procedures, in particular the personnel of seed testing laboratories and regulatory agencies; seed producers and farmers. The updated publication represents a significant improvement over previous guidelines, as it incorporates advances in technology and provides guidance on a broader range of testing procedures. The guidelines are designed to be adaptable to different contexts and seed types and include recommendations for quality management systems, proficiency testing, and inter-laboratory comparisons, which are essential for ensuring the accuracy and reliability of seed testing results.

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