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Isolation of DNA from preserved woods for identification of wood species through genetic analysis regardless of the age of wood production

XV World Forestry Congress, 2-6 May 2022

Year of publication 2022

Place of publication Rome, Italy ;

Pages 1p.

Author Lee, J., Kim, T.-J.

Publisher FAO ;

Product type Document

Synopsis (short abstract) Species identification of wood provides important information for archaeology, restoration of cultural assets, preventing illegal logging, and more. Wood species are usually identified based on their anatomical features with the use of a microscope. However, this method may not be able to distinguish between anatomically similar species or subspecies. To overcome this problem, wood species need to be identified at the molecular level using DNA sequencing. However, unlike living plant cells, wood is difficult to pulverize using a mortar, and DNA extraction from dried wood is challenging. To solve these problems, we propose a pretreatment method in which wood is pulverized using 60-grit sandpaper and hydrated with water for 2 days. Using this method, we were able to stably amplify the rpoB gene from the extracted DNA of Pinus rigida. In addition, sequence analysis of the rpoB gene revealed six single nucleotide polymorphisms (SNPs), which classified the rpoB sequences in the genus Pinus into five groups. Our data indicate that although these SNPs were not suitable for species identification, they can potentially be used to determine the origin of different wood subspecies or individual samples of wood. Keywords: Monitoring and data collection, Research ID: 3616978

Statutory body / Meeting title World Forestry Congress (WFC)

Language English

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    Article
    The preservation of natural forests through the establishment of industrial wood production plantations via the new, fast growing Robinia pseudoacacia ‘Turbo Obelisk’ varieties and new cultivation technologies
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    Also available in:
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