Year of publication 2020

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Place of publication Bangkok, Thailand ;

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Pages 8p.

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Author FAO

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Publisher FAO ;

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Product type Brochure, flyer, fact-sheet

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Edition 1

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Synopsis (short abstract) The Food and Agriculture Organization of the United Nations (FAO) and the World Organisation for Animal Health (OIE) including other partners have been working in countries affected or at risk of incursion by African swine fever (ASF). This document was generated as guidance in response to the emergence of ASF in China, Southeast Asia, and the Pacific. FAO has provided support for laboratory diagnosis of ASF following OIE recommendations, specifically using Polymerase Chain Reaction (PCR) in detecting ASF virus. PCR is a highly sensitive and specific method for the molecular detecting ASF virus for a wide range of purposes, including confirmation of clinical cases and confirmation of freedom from infection before movement. The Australian Centre for Disease Preparedness (ACDP, formerly the Australian Animal Health Laboratories) has developed a diagnostic algorithm based on OIE recommendations and in consultation with the Association of Southeast Asian Nations (ASEAN) regional animal health laboratory network. This document describes a validated real time reverse transcription-polymerase chain reaction (RT-PCR) protocol (the ‘King assay’), which targets the B646L gene, encoding the ASF virus structural protein p72. This assay has been produced in kit form by the ACDP and provided to various veterinary diagnostic laboratories in Southeast Asia by the FAO and OIE. This document also provides links to other reference documents.

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Language English

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