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Standard operating procedure for soil available phosphorus - Bray I and Bray II method












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    Standard operating procedure for soil available phosphorus - Mehlich I Method 2021
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    Mehlich I method is suitable for determining bioavailable P in acid soils with low cation exchange capacity; (CEC) less than 10 cmolc kg-1. The amount of P and K extracted with Mehlich-1 has been calibrated to quantify the amount of P and K fertilizer needed to maximize crop yields in various regions. This method has also been adapted for simultaneous determination of additional plant nutrients in the extract using ICP-OES.
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    Standard operating procedure for soil available phosphorus - Olsen method 2021
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    This procedure is suitable for calcareous, alkaline, neutral, and slightly acidic soils containing CaPO4, since the calcium concentration in the solution is suppressed by the precipitation of CaCO3, increasing PO4 concentration in solution. Neutral and slightly acid soils (pH 6.0 to 7.0) may contain both Ca- and Al-phosphates. The NaHCO3 extractant can remove Ca-phosphates and phosphate adsorbed on the surface of calcium and magnesium carbonates along with Al-phosphates and is considered the most suitable extractant for these soils.
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    Standard operating procedure for soil organic carbon: Tyurin spectrophotometric method 2021
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    This protocol applies to the determination of the oxidizable organic carbon content in the soil. The organic carbon content is calculated from the amount of chromic ion (Cr3+) formed which is determined by the spectrophotometric method.

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