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Standard operating procedure for soil available phosphorus - Mehlich I Method











FAO. 2021. Standard operating Procedure for soil available phosphorus - Mehlich I method. Rome. 


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    This procedure is suitable for calcareous, alkaline, neutral, and slightly acidic soils containing CaPO4, since the calcium concentration in the solution is suppressed by the precipitation of CaCO3, increasing PO4 concentration in solution. Neutral and slightly acid soils (pH 6.0 to 7.0) may contain both Ca- and Al-phosphates. The NaHCO3 extractant can remove Ca-phosphates and phosphate adsorbed on the surface of calcium and magnesium carbonates along with Al-phosphates and is considered the most suitable extractant for these soils.
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    P-Bray 1 and P-Bray 2 methods are normally limited to acid soils with water pH values less than 6.8. The P-Bray 1 Method removes a fraction of the “adsorbed” phosphorus (Al-P, Fe-P, Mn-P and Ca-P but less efficient) while the P-Bray 2 Method is best suited to acid soils where rock phosphate has been the primary P fertilizer source and/or the major portion of P exists in the soil in various forms of calcium phosphate. Bray extractants should not be used on alkaline soils because the acid tends to be neutralized and/or excessive calcium phosphates may be extracted, giving a false high test for available P.
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    This Standard Operating Procedure (SOP) reports how to determine soil respiration rate, which is one of the longest established and most frequently used parameter for quantifying microbial activity in soils. It is defined as oxygen (O2) uptake or carbon dioxide (CO2) evolution by soil microorganisms and includes the gas exchange of aerobic and anaerobic metabolism.

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