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Standard operating procedure for saturated soil paste extract









​FAO. 2021. Standard operating procedure for saturated soil paste extract. Rome. 


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    This method determines the electrical conductivity (EC) of a soil/water suspension in the ratio of one:five (1:5). Aqueous extracts of soil samples are usually made at higher than normal water content for routine characterization purposes, as obtaining soil samples at typical field water contents are not very practical. Considering that the amounts of various solutes are influenced by the soil/water ratio at which the extract is made, the soil:water ratio should be standardized to obtain results that can be applied and reasonably interpreted.
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    The method provides a potential indicator of the plant available copper, iron, manganese and zinc, which are essential soil micronutrients for plant growth and living organisms. It can be used to extract heavy metals such as nickel, lead and cadmium assisting with conclusions as to their potential bioavailability. This method must be used in combination with suitable determination methods, for example, flame atomic absorption spectrometry, electrothermal atomic absorption spectrometry or inductively coupled plasma – optical emission spectrometry.
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    The hot water-soluble B procedure is the most commonly used method for predicting the concentration of B that will be available to plants in a soil solution. Hot water-soluble B is commonlyused because the water-soluble B extracted by the hot water method correlates positively to plant growth response, as it measures B from the adsorbed, organic, and soluble pools of the soil at boiling temperatures. The aim of this SOP is to guide users on the determination of hot water-soluble B in soils through the application of three routine procedures, using azomethine-H, AAS, ICP-AES, and the Berger and Truog extraction method.

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