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Standard operating procedure for saturated soil paste extract









​FAO. 2021. Standard operating procedure for saturated soil paste extract. Rome. 


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    Standard operating procedure for soil electrical conductivity (soil/water, 1:5) 2021
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    This method determines the electrical conductivity (EC) of a soil/water suspension in the ratio of one:five (1:5). Aqueous extracts of soil samples are usually made at higher than normal water content for routine characterization purposes, as obtaining soil samples at typical field water contents are not very practical. Considering that the amounts of various solutes are influenced by the soil/water ratio at which the extract is made, the soil:water ratio should be standardized to obtain results that can be applied and reasonably interpreted.
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    The method provides a potential indicator of the plant available copper, iron, manganese and zinc, which are essential soil micronutrients for plant growth and living organisms. It can be used to extract heavy metals such as nickel, lead and cadmium assisting with conclusions as to their potential bioavailability. This method must be used in combination with suitable determination methods, for example, flame atomic absorption spectrometry, electrothermal atomic absorption spectrometry or inductively coupled plasma – optical emission spectrometry.
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    Standard operating procedure for soil microbial biomass (carbon): chloroform fumigation-extraction method 2024
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    Microbial biomass is considered as an estimation of soil biological activities and the capacity to mediate soil biochemical reactions. It is the most dynamic and labile of soil organic matter fractions, generally accounting for 1 to 5 percent of soil organic matter and very sensitive to soil management. The need to quantify soil microbiota has become increasingly relevant in current times as they are responsible for many different processes like the degradation of organic matter, the stability of aggregates and most of the nutrient cycling that occurs in soils. This standard operating procedure (SOP) focuses on the determination of soil microbial biomass using the chloroform fumigation-extraction method, which is applicable to both aerobic and anaerobic conditions over the whole range of soil pH, regardless of land use type. While chloroform fumigation also affects soil fauna, the carbon aliquot derived from these organisms is generally small (less than 5 percent) and can usually be disregarded.

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