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Wood transcriptome profiling identifies critical pathway genes of secondary wall biosynthesis and novel regulators for vascular cambium development in populus

XV World Forestry Congress, 2-6 May 2022










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    Wood transcriptome analysis of Pinus densiflora identifies genes critical for secondary cell wall formation and NAC transcription factors involved in tracheid formation
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Although conifers have significant ecological and economic value, information on transcriptional regulation of wood formation in conifers is still limited. Here, to gain insight into secondary cell wall (SCW) biosynthesis and tracheid formation in conifers, we performed wood tissue-specific transcriptome analyses of Pinus densiflora (Korean red pine) using RNA sequencing. In addition, to obtain full-length transcriptome information, PacBio single molecule real-time (SMRT) iso-sequencing was carried out using RNAs from 28 tissues of P. densiflora. Subsequent comparative tissue-specific transcriptome analysis successfully pinpointed critical genes encoding key proteins involved in biosynthesis of the major secondary wall components (cellulose, galactoglucomannan, xylan, and lignin). Furthermore, we predicted a total of 62 NAC (NAM, ATAF1/2 and CUC2) family transcription factor members and identified seven PdeNAC genes preferentially expressed in developing xylem tissues in P. densiflora. Protoplast-based transcriptional activation analysis found that four PdeNAC genes, homologous to VND, NST and SND/ANAC075, upregulated GUS activity driven by an SCW-specific cellulose synthase promoter. Consistently, transient overexpression of the four PdeNACs induced xylem vessel cell-like SCW deposition in both tobacco (Nicotiana benthamiana) and Arabidopsis leaves. Taken together, our data provide a foundation for further research to unravel transcriptional regulation of wood formation in conifers, especially SCW formation and tracheid differentiation. Keywords: Research, Genetic resources ID: 3622610
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    Enhanced drought tolerance by expression of hvDhn5 gene in poplar
    XV World Forestry Congress, 2-6 May 2022
    2022
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    We transferred Dhn5(dehydrin5) gene from barley to poplar to determine the effect of its expression on the transgenic poplars. The results from northern blot analysis showed that the expression level of gene varied among the transgenic lines. During their culture on tissue culture media, the transgenic poplars formed vigorous growing callus in the presence of 5% PEG. When the transgenic poplars were growing in pots and withheld watering, they stayed much healthier than nontransgenic poplars. The transgenic poplars showed higher rates of photosynthetic rates, stomatal conductance and evaporation rates under the drought stress, although there was no significant difference in soil water content within the treatments. The relative electrical conductivity of the transgenic poplars after 20% PEG treatment was lower than that of nontransgenic poplars. The results provide evidence that the expression of hvDhn5 gene conferred drought tolerance in the transgenic poplars. Keywords : Dehydrin5, Drought tolerance, Photosynthetic rates, Relative electrical conductivity, Transgenic poplar ID: 3622412
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    What is RNA interference (RNAi) and how this can be relevant to our food?
    A Seminar by Nobel Laureate, Dr. Andrew Fire
    2015
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    Dr Fire spoke about how RNAi was discovered. Starting from the first unexpected observations that exogenous RNA fragments can inhibit the expression of complementary genes in Fungi, Plants, and Caenorhabditis elegans, Dr Fire elaborated on how the underlying molecular processes of this process were subsequently further unraveled. In the second part of his presentation Dr Fire specifically addressed the potential applications of RNAi in the food and agricultural sector.

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