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Wood transcriptome analysis of Pinus densiflora identifies genes critical for secondary cell wall formation and NAC transcription factors involved in tracheid formation

XV World Forestry Congress, 2-6 May 2022










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    Wood transcriptome profiling identifies critical pathway genes of secondary wall biosynthesis and novel regulators for vascular cambium development in populus
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Wood, the most abundant biomass on Earth, is composed of secondary xylem differentiated from vascular cambium. However, the underlying molecular mechanisms of wood formation remain largely unclear. To gain insight into wood formation, we performed a series of wood-forming tissue-specific transcriptome analyses from a hybrid poplar (Populus alba × P. glandulosa, clone BH) using RNA-seq. Together with shoot apex and leaf tissue, cambium and xylem tissues were isolated from vertical stem segments representing a gradient of secondary growth developmental stages (i.e., immature, intermediate, and mature stem). In a comparative transcriptome analysis of the ‘developing xylem’ and ‘leaf’ tissue, we could identify critical players catalyzing each biosynthetic step of secondary wall components (e.g., cellulose, xylan, and lignin). Several candidate genes involved in the initiation of vascular cambium formation were found via a co-expression network analysis using abundantly expressed genes in the ‘intermediate stem-derived cambium’ tissue. We found that transgenic Arabidopsis plants overexpressing the PtrHAM4-1, a GRAS family transcription factor, resulted in a significant increase of vascular cambium development. This phenotype was successfully reproduced in the transgenic poplars overexpressing the PtrHAM4-1. Taken together, our results may serve as a springboard for further research to unravel the molecular mechanism of wood formation, one of the most important biological processes on this planet. Keywords: Genetic resources, Research ID: 3622616
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    The early growth performances of Pinus densiflora and Larix kaempferi seedlings under open-field experimental warming and precipitation manipulation
    XV World Forestry Congress, 2-6 May 2022
    2022
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    This study aimed to investigate the effects of climate change on the survival and growth performance of Pinus densiflora and Larix kaempferi seedlings using open-field experimental warming and precipitation manipulation. We measured the survival rate, root-collar diameter, and height, and then calculated the seedling quality index (SQI) of 2-year-old seedlings under 6 treatments [2 temperatures (TC: Control; TW: Warming) × 3 precipitation manipulations (PC: Control; PD: Decreased; PI: Increased)] and performed a two-way ANOVA to test for differences. The air temperature of the warming plots was 3°C higher than that of the control plots, while the precipitation manipulation plots received ±40% of the precipitation received by the control plots. Temperature and precipitation treatments did not significantly affect the survival rate of P. densiflora; however, the SQI of P. densiflora decreased with increasing precipitation. In contrast, the mortality rate of L. kaempferi increased with increasing temperature and decreasing precipitation. Furthermore, in L. kaempferi, TC × PI treatment resulted in the lowest SQI with a significant interaction effect observed between the two factors. In summary, low seedling production and quality should be expected in P. densiflora as precipitation increases and in L. kaempferi as temperature increases or precipitation decreases. These results indicate species-specific sensitivities to climate change of two plant species at the nursery stage. With the occurrence of global warming, the frequencies of drought and heavy rainfall events are increased, and this could affect the survival and seedling quality of tree species. Therefore, it is necessary to improve nursery techniques by establishing new adaptation strategies based on species-specific growth performance responses. 1) Keywords: Climate change ID: 3622385
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    Characterization of formate dehydrogenase from Trametes versicor for formate production from CO2 gas
    XV World Forestry Congress, 2-6 May 2022
    2022
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    Enzymatic CO2 reduction has been reported as a promising approach to greenhouse gas fixation. There are few biocatalysts capable of biological CO2 fixation, e.g. pyruvate decarboxylase, carbonic anhydrase, and formate dehydrogenase. Formate dehydrogenase(FDH) can reduce CO2 to formate without organic chemicals, and formate can be sequentially reduced to formaldehyde and methanol by coupling aldehyde dehydrogenase and alcohol dehydrogenase reactions. Therefore, FDH has been widely adopted inCO2 reducing reaction.
    In this study, we researched CO2-reducing activities of FDH derived from wood rot fungi, Trametes versicolor. Microbial species, T. versicolor, were separated in National institute of Forest Science. The fungi were grown in a potato dextrose agar medium. For the transformation, the host, Shizosaccaromyces pombe of wild type, was obtained from Bioneer(Korea). Total RNA was extracted with Hybrid-R (GeneAll) from T. versicolor mycelium. cDNAs were synthezied by AccuPower (Bioneer) as recommended by the manufacturer. For sub-cloning, PCR of fragments (1.2 kb) were amplified with pairs of TvFDH ORF primers(Foword: 5’-ATGCTCGCCGGCATCT-3’, Reverse: 5’-TCACTTGCGCTGGCCA-3’). The amplified fragments were sub-cloned into a pCR2.1-TOPO (Invitrogen, Carlsbad, CA, USA) vector and the plasmid DNA was sequenced. The amplified fragments were digested by Not 1 and Bam 1 and then inserted into a pSLF272 vector (Bioneer, Korea). The recombinant plasmid was amplified in the Escherichia coli DH5α, and then extracted by mini prep kit. The recombinant plasmids were chemically (lithium acetate method) transformed into yeast S. pombe. Ligation products, transformants, were named as pSLF272-TvFDH. Selection of transformants were performed in the minimal medium, EMM. Expression and purification of the TvFDHs is being performed to obtain pure enzymes. Keywords: Climate change, Research, Genetic resources, Agriculture, Innovation ID: 3604933

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